Nano-LEDs emit full visible spectrum of light

Nano-LEDs emit full visible spectrum of light

  2:04:00 AM    Science Lover

---

Physicists through Taiwan possess designed along with fabricated nano-sized light-emitting diodes (LEDs) that emit light source spanning your whole visible spectrum. Although all the tiny full-color LEDs aren't meant for commercial lighting fixtures applications, they must be useful within high-resolution microscopy and additionally subwavelength photolithography.

A single nanodisk-nanorod LED viewed with a field-emission
scanning electron microscope. (Right) Some colors of light
emissions from nanodisk-nanorod LEDs - violet, blue, cyan,
green, and yellow - viewed with an optical microscope.
Image credit: Lu, et al. ©2011 American Institute of Physics

A researchers, Yu-Jung Lu, et ing., from National Tsing-Hua University or college in Hsinchu, Taiwan, have published their study to the nano-LEDs in a the latest issue of Applied Physics Letters.

The new nano-LEDs have a very good unique structure that is made of 40-nm-thick nanodisks sandwiched involving two layers of nanorods, creating a nanodisk-in-nanorod geometry. The nanodisks are made from indium gallium nitride (InGaN), a semiconducting material that is definitely widely used in LEDs and solar cells, while the nanorods are made from gallium nitride (GaN). Having said that, InGaN LEDs capable of emitting light belonging to the entire visible spectrum haven’t been achieved until now.



“The InGaN/GaN nanodisk/nanorod structure is just like a well-known quantum well structure, but in a lower dimensionality (reduction during lateral sizes), ” coauthor Shangjr Gwo, your physics professor at National Tsing-Hua University, told PhysOrg. com. “The InGaN nanodisks sandwiched regarding the p- and n-GaN regions be working as the full-color visible-light emitters anytime electrons and holes are injected through the p-n junction at a forward bias voltage. The electroluminescent light hails from the electron-hole recombination during the InGaN nanodisks. ”

As being the researchers explained, the essential to achieving full-color LEDs was first overcoming large lattice traces, which degrade long-wavelength emissions. The InGaN/GaN nanorod method resolves this issue as a consequence of strain relaxation in all the nanostructured geometry.

The researchers hope the full-color nano-LEDs work extremely well in high-resolution imaging techniques which could resolve ultrasmall subwavelength features of objects. To do this approach, these techniques must overcome the diffraction limit, the fundamental limit on imaging resolution as a result of the spreading out – and / or “diffraction” – of surf. Imaging techniques can find their way this limit by employing evanescent waves, which reveal home elevators objects’ subwavelength features, and decay exponentially away through the object. Due to the short range of the evanescent waves, imaging techniques that detect them depend on near-field optics.

One of such techniques is scanning near-field optical microscopy (SNOM), which operates on the all tiny probe to acquire and retrieve evanescent ocean. One of the biggest challenges in SNOM gets a light source that could be small and versatile enough to function on this probe, and that’s where new nano-LEDs come for. While previous research has demonstrated learn about using nano-LEDs on your probes, this is to start with that a nano-LED using a full-color range has already been available.

“For microscopy, we will use the nano-LED as being a localized excitation light source with a chosen wavelength to selectively inspire specific fluorescent molecules, ” Lu reported.

In their study, the researchers experimentally demonstrated making use of the nanodisk-in-nanorod LEDs for subwavelength photolithography, in which light is used to generate a pattern on a light-sensitive materials. They predict that, by way of fabricating the nano-LEDs upon the SNOM probe suggestions, they could achieve far better spatial control for near future subwavelength photolithography.

“For a applications of photolithography, the freedom of utilizing nano-LEDs at any wavelength broadens the options of photoresist and consists of the control of most of the photo-response, ” Lu reported.

More information: Yu-Jung Lu, et ing. “Single InGaN nanodisk lumination emitting diodes as full-color subwavelength lighting sources. ” Applied Physics Albhabets. DOI: 10. 1063/1. 3597211.

Read More

Microscope 'Sees' Atoms for First Time

Microscope 'Sees' Atoms for First Time

  9:16:00 PM    Science Lover

---

UCLA researchers report in the April 30 edition of the journal Cell that they have imaged a virus structure at a resolution high enough to effectively "see" atoms, the first published instance of imaging biological complexes at such a resolution.

Cover of the journal Cell showing an artistic 
representation of a Cryo-EM 3-D reconstruction 
of an aquareovirus, the colors are added to show 
contrast between various structures in the virus. 
(Credit: Image courtesy of UCLA)

The research team, led by Hong Zhou, UCLA professor of microbiology, immunology and molecular genetics, used cryo-electron microscopy to image the structure at 3.3 angstroms. An angstrom is the smallest recognized division of a chemical element and is about the distance between the two hydrogen atoms in a water molecule.

The study, the researchers say, demonstrates the great potential of cryo-electron microscopy, or Cryo-EM, for producing extremely high-resolution images of biological samples in their native environment.

"This is the first study to determine an atomic resolution structure through Cryo-EM alone," said Xing Zhang, a postdoctoral candidate in Zhou's group and lead author of the Cell paper. "By proving the effectiveness of this microscopy technique, we have opened the door to a wide variety of biological studies."

With traditional light microscopy, a magnified image of a sample is viewed through a lens. Some samples, however, are too small to diffract visible light (in the 500 to 800 nm range, or 5,000 to 8,000 angstroms) and therefore cannot be seen. To image objects at the sub-500 nm scale, scientists must turn to other tools, such as atomic force microscopes, which use an atomically thin tip to generate an image by probing a surface, in much the same way a blind person reads by touching Braille lettering.

With electron microscopy, another sub-500 nm technology, a beam of electrons is fired at a sample, passing through empty areas and bouncing off dense areas. A digital camera reads the path of the electrons passing through the sample to create a two-dimensional projection image of the sample. By repeating this process at hundreds of different angles, a computer can construct a three-dimensional image of the sample at a very high resolution.

Zhou is faculty director of the Electron Imaging Center for Nanomachines (EICN) at UCLA's California NanoSystems Institute, which is using cryo-electron microscopy to create 3-D reconstructions of nano-machineries, nano-devices and biological nano-structures, such as viruses.

Structurally accurate 3-D reconstructions of biological complexes are possible with cryo-electron microscopy because the samples are flash frozen, which allows them to be imaged in their native environment, and the microscope operates in a vacuum, because electrons travel better in that environment. The Cell paper focused on a structural study of the aquareovirus, a non-envelope virus that causes disease in fish and shellfish, in an effort to better understand how non-envelope viruses infect host cells.

"We are extremely excited about the recent breakthrough achieved by Hong Zhou and his team at the EICN lab," said Leonard H. Rome, senior associate dean for research at the David Geffen School of Medicine at UCLA and associate director of the California NanoSystems Institute. "The ability to understand the structure of viruses at an atomic level will open avenues for manipulating them for use in drug delivery and propel numerous innovations in treatments of diseases. UCLA is fortunate to have such specialized instrumentation and the expertise of Professor Zhou and his team to take advantage of these marvelous microscopes."

Viruses can be classed into two types: envelope and non-envelope. Envelope viruses, which include influenza and HIV, are surrounded by an envelope-like membrane which the virus uses to fuse with and infect a host cell. Non-envelope viruses lack this membrane and instead use a protein to fuse with and infect cells. This process was poorly understood until Zhou's study.

"Through better knowledge of virus structures, we hope to engineer medications in three ways," Zhou said. "If we understand how viruses work, first we can identify small molecules or drugs that block their infection; second, we can engineer ultra-stable and non-infectious virus-like particles as optimal vaccines; and third, we can alter their characteristics so that instead of delivering a disease, viruses could deliver medications.

"Indeed, we are working with UCLA physicians and engineers to engineer viruses for gene therapy and drug delivery," he said. "In essence, we hope to take advantage of millions of years of evolution that have made viruses incredibly effective delivery platforms."

From the high-resolution 3-D images produced with the cryo-electron microscopy, Zhou's group was able to determine that the aquareovirus employs a priming stage to accomplish cell infection. In its dormant state, the virus has a protective protein covering, which it sheds during priming. Once the outer shell has been shed, the virus is in a primed state and is ready to use a protein called an "insertion finger" to infect a cell.

The team's study ushers in a new era of structural biology for understanding important biological processes. The group was able to discover this functionality because of the accurate structural model produced through cryo-electron microscopy. In addition to producing a high-resolution 3-D image of samples, the technology allows samples to be imaged in their native environment, so the structural model is faithful to the original sample. From a technical point of view, this work also demonstrates the power of cryo-electron microscopy in obtaining 3-D structures of biological complexes without needing to grow a crystal.

The California NanoSystems Institute at UCLA is an integrated research center operating jointly at UCLA and UC Santa Barbara whose mission is to foster interdisciplinary collaborations for discoveries in nanosystems and nanotechnology; train the next generation of scientists, educators and technology leaders; and facilitate partnerships with industry, fueling economic development and the social well-being of California, the United States and the world. The CNSI was established in 2000 with $100 million from the state of California and an additional $250 million in federal research grants and industry funding. At the institute, scientists in the areas of biology, chemistry, biochemistry, physics, mathematics, computational science and engineering are measuring, modifying and manipulating the building blocks of our world -- atoms and molecules. These scientists benefit from an integrated laboratory culture enabling them to conduct dynamic research at the nanoscale, leading to significant breakthroughs in the areas of health, energy, the environment and information technology.

Read More

World's smallest periscopes

World's smallest periscopes

  2:05:00 AM    Science Lover

---

A team of scientists has designed the world's tiniest version of the periscope to peer at cells and other micro-organisms from all the sides at once.

"With an off-the-shelf laboratory microscope you only see cells from one side, the top," said Chris Janetopoulos, assistant professor of biological sciences at the Vanderbilt University (VU) and member of the research team.

"Now not only can we see the tops of cells, we can view their sides as well - something biologists almost never see," he added.

The researchers dubbed their devices "mirrored pyramidal wells". They consist of pyramidal-shaped cavities moulded into silicon whose interior surfaces are coated with a reflective layer of gold or platinum.

They are about the width of a human hair and can be made in a range of sizes to view different-sized objects. When a cell is placed in such a well and viewed with a regular microscope, the researcher can see several sides simultaneously.

"This technology is exciting because these mirrored wells can be made at very low cost, unlike other, more complex methods for 3D microscopy," said VU assistant professor Kevin Seale.

"This could easily become as ubiquitous as the microscope slide and could replace more expensive methods currently used to position individual cells," said Ron Reiserer, lab manager at the Vanderbilt Institute for Integrative Biosystems Research and Education (VIIBRE) who helped design the protocol used to make the micropyramids.

The Vanderbilt group is not the first to make microscopic pyramidal wells, but it is the first to apply them to make 3D images of microorganisms, said a Vanderbilt release.

These findings were published in the Journal of Microscopy.

Read More