Blood Mystery Solved: Two New Blood Types Identified

Blood Mystery Solved: Two New Blood Types Identified

  10:20:00 AM    Science Lover

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You probably know your blood type: A, B, AB or O. You may even know if you're Rhesus positive or negative. But how about the Langereis blood type? Or the Junior blood type? Positive or negative? Most people have never even heard of these.

Blood samples. You probably know your blood type: A, B, 
AB or O. You may even know if you're Rhesus positive or 
negative. But how about the Langereis blood type? Or the 
Junior blood type? Positive or negative? Most people have 
never even heard of these. (Credit: © weim / Fotolia)

Yet this knowledge could be "a matter of life and death," says University of Vermont biologist Bryan Ballif.

While blood transfusion problems due to Langereis and Junior blood types are rare worldwide, several ethnic populations are at risk, Ballif notes. "More than 50,000 Japanese are thought to be Junior negative and may encounter blood transfusion problems or mother-fetus incompatibility," he writes.

But the molecular basis of these two blood types has remained a mystery -- until now.

In the February issue of Nature Genetics, Ballif and his colleagues report on their discovery of two proteins on red blood cells responsible for these lesser-known blood types.

Ballif identified the two molecules as specialized transport proteins named ABCB6 and ABCG2.

"Only 30 proteins have previously been identified as responsible for a basic blood type," Ballif notes, "but the count now reaches 32."

The last new blood group proteins to be discovered were nearly a decade ago, Ballif says, "so it's pretty remarkable to have two identified this year."

Both of the newly identified proteins are also associated with anticancer drug resistance, so the findings may also have implications for improved treatment of breast and other cancers.

As part of the international effort, Ballif, assistant professor in the biology department, used a mass spectrometer at UVM funded by the Vermont Genetics Network. With this machine, he analyzed proteins purified by his longtime collaborator, Lionel Arnaud at the French National Institute for Blood Transfusion in Paris, France.

Ballif and Arnaud, in turn, relied on antibodies to Langereis and Junior blood antigens developed by Yoshihiko Tani at the Japanese Red Cross Osaka Blood Center and Toru Miyasaki at the Japanese Red Cross Hokkaido Blood Center.

After the protein identification in Vermont, the work returned to France. There Arnaud and his team conducted cellular and genetic tests confirming that these proteins were responsible for the Langereis and Junior blood types. "He was able to test the gene sequence," Ballif says, "and, sure enough, we found mutations in this particular gene for all the people in our sample who have these problems."

Transfusion troubles

Beyond the ABO blood type and the Rhesus (Rh) blood type, the International Blood Transfusion Society recognizes twenty-eight additional blood types with names like Duffy, Kidd, Diego and Lutheran. But Langereis and Junior have not been on this list. Although the antigens for the Junior and Langereis (or Lan) blood types were identified decades ago in pregnant women having difficulties carrying babies with incompatible blood types, the genetic basis of these antigens has been unknown until now.

Therefore, "very few people learn if they are Langereis or Junior positive or negative," Ballif says.

"Transfusion support of individuals with an anti-Lan antibody is highly challenging," the research team wrote in Nature Genetics, "partly because of the scarcity of compatible blood donors but mainly because of the lack of reliable reagents for blood screening." And Junior-negative blood donors are extremely rare too. That may soon change.

With the findings from this new research, health care professionals will now be able to more rapidly and confidently screen for these novel blood group proteins, Ballif wrote in a recent news article. "This will leave them better prepared to have blood ready when blood transfusions or other tissue donations are required," he notes.

"Now that we know these proteins, it will become a routine test," he says.

A better match

This science may be especially important to organ transplant patients. "As we get better and better at transplants, we do everything we can to make a good match," Ballif says. But sometimes a tissue or organ transplant, that looked like a good match, doesn't work -- and the donated tissue is rejected, which can lead to many problems or death.

"We don't always know why there is rejection," Ballif says, "but it may have to do with these proteins."

The rejection of donated tissue or blood is caused by the way the immune system distinguishes self from not-self. "If our own blood cells don't have these proteins, they're not familiar to our immune system," Ballif says, so the new blood doesn't "look like self" to the complex cellular defenses of the immune system. "They'll develop antibodies against it," Ballif says, and try to kill off the perceived invaders. In short, the body starts to attack itself.

"Then you may be out of luck," says Ballif, who notes that in addition to certain Japanese populations, European Gypsies are also at higher risk for not carrying the Langereis and Junior blood type proteins.

"There are people in the United States who have these challenges too," he says, "but it's more rare."

Other proteins

Ballif and his international colleagues are not done with their search. "We're following up on more unknown blood types," he says. "There are probably on the order of 10 to 15 more of these unknown blood type systems -- where we know there is a problem but we don't know what the protein is that is causing the problem."

Although these other blood systems are very rare, "if you're that one individual, and you need a transfusion," Ballif says, "there's nothing more important for you to know."

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Replacing Electricity With Light: First Physical 'Metatronic' Circuit Created

Replacing Electricity With Light: First Physical 'Metatronic' Circuit Created

  8:15:00 PM    Science Lover

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The technological world of the 21^st century owes a tremendous amount to advances in electrical engineering, specifically, the ability to finely control the flow of electrical charges using increasingly small and complicated circuits. And while those electrical advances continue to race ahead, researchers at the University of Pennsylvania are pushing circuitry forward in a different way, by replacing electricity with light.

Figure A. When the plane of the electric field is
in line with the nanorods the circuit is wired in
parallel. Figure B. When the plane of the electric
field crosses both the nanorods and the gaps the
circuit is wired in series. (Credit: Image courtesy
of University of Pennsylvania)

"Looking at the success of electronics over the last century, I have always wondered why we should be limited to electric current in making circuits," said Nader Engheta, professor in the electrical and systems engineering department of Penn's School of Engineering and Applied Science. "If we moved to shorter wavelengths in the electromagnetic spectrum -- like light -- we could make things smaller, faster and more efficient."

Different arrangements and combinations of electronic circuits have different functions, ranging from simple light switches to complex supercomputers. These circuits are in turn built of different arrangements of circuit elements, like resistors, inductors and capacitors, which manipulate the flow of electrons in a circuit in mathematically precise ways. And because both electric circuits and optics follow Maxwell's equations -- the fundamental formulas that describe the behavior of electromagnetic fields -- Engheta's dream of building circuits with light wasn't just the stuff of imagination. In 2005, he and his students published a theoretical paper outlining how optical circuit elements could work.

Now, he and his group at Penn have made this dream a reality, creating the first physical demonstration of "lumped" optical circuit elements. This represents a milestone in a nascent field of science and engineering Engheta has dubbed "metatronics."

Engheta's research, which was conducted with members of his group in the electrical and systems engineering department, Yong Sun, Brian Edwards and Andrea Alù, was published in the journal Nature Materials.

In electronics, the "lumped" designation refers to elements that can be treated as a black box, something that turns a given input to a perfectly predictable output without an engineer having to worry about what exactly is going on inside the element every time he or she is designing a circuit.

"Optics has always had its own analogs of elements, things like lenses, waveguides and gratings," Engheta said, "but they were never lumped. Those elements are all much larger than the wavelength of light because that's all that could be easily built in the old days. For electronics, the lumped circuit elements were always much smaller than the wavelength of operation, which is in the radio or microwave frequency range."

Nanotechnology has now opened that possibility for lumped optical circuit elements, allowing construction of structures that have dimensions measured in nanometers. In this experiment's case, the structure was comb-like arrays of rectangular nanorods made of silicon nitrite.

The "meta" in "metatronics" refers to metamaterials, the relatively new field of research where nanoscale patterns and structures embedded in materials allow them to manipulate waves in ways that were previously impossible. Here, the cross-sections of the nanorods and the gaps between them form a pattern that replicates the function of resistors, inductors and capacitors, three of the most basic circuit elements, but in optical wavelengths.

"If we have the optical version of those lumped elements in our repertoire, we can actually make designs similar to what we do in electronics but now for operation with light," Engheta said. "We can build a circuit with light."

In their experiment, the researchers illuminated the nanorods with an optical signal, a wave of light in the mid-infrared range. They then used spectroscopy to measure the wave as it passed through the comb. Repeating the experiment using nanorods with nine different combinations of widths and heights, the researchers showed that the optical "current" and optical "voltage" were altered by the optical resistors, inductors and capacitors with parameters corresponding to those differences in size.

"A section of the nanorod acts as both an inductor and resistor, and the air gap acts as a capacitor," Engheta said.

Beyond changing the dimensions and the material the nanorods are made of, the function of these optical circuits can be altered by changing the orientation of the light, giving metatronic circuits access to configurations that would be impossible in traditional electronics.

This is because a light wave has polarizations; the electric field that oscillates in the wave has a definable orientation in space. In metatronics, it is that electric field that interacts and is changed by elements, so changing the field's orientation can be like rewiring an electric circuit.

When the plane of the field is in line with the nanorods, as in Figure A, the circuit is wired in parallel and the current passes through the elements simultaneously. When the plane of the electric field crosses both the nanorods and the gaps, as in Figure B, the circuit is wired in series and the current passes through the elements sequentially.

"The orientation gives us two different circuits, which is why we call this 'stereo-circuitry,'" Engheta said. "We could even have the wave hit the rods obliquely and get something we don't have in regular electronics: a circuit that's neither in series or in parallel but a mixture of the two."

This principle could be taken to an even higher level of complexity by building nanorod arrays in three dimensions. An optical signal hitting such a structure's top would encounter a different circuit than a signal hitting its side. Building off their success with basic optical elements, Engheta and his group are laying the foundation for this kind of complex metatronics.

"Another reason for success in electronics has to do with its modularity," he said. "We can make an infinite number of circuits depending on how we arrange different circuit elements, just like we can arrange the alphabet into different words, sentences and paragraphs.

"We're now working on designs for more complicated optical elements," Engheta said. "We're on a quest to build these new letters one by one."

This work was supported in part by the U.S. Air Force Office of Scientific Research.

Andrea Alù is now an assistant professor at the University of Texas at Austin.

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More Powerful Electric Cars: Mechanism Behind Capacitor's High-Speed Energy Storage Discovered

More Powerful Electric Cars: Mechanism Behind Capacitor's High-Speed Energy Storage Discovered

  2:33:00 AM    Science Lover

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Researchers at North Carolina State University have discovered the means by which a polymer known as PVDF enables capacitors to store and release large mounts of energy quickly. Their findings could lead to much more powerful and efficient electric cars.

Researchers have discovered the means by which a polymer
known as PVDF enables capacitors to store and release large
amounts of energy quickly. Their findings could lead to
much more powerful and efficient electric cars.
(Credit: iStockphoto)

Capacitors are like batteries in that they store and release energy. However, capacitors use separated electrical charges, rather than chemical reactions, to store energy. The charged particles enable energy to be stored and released very quickly. Imagine an electric vehicle that can accelerate from zero to 60 miles per hour at the same rate as a gasoline-powered sports car. There are no batteries that can power that type of acceleration because they release their energy too slowly. Capacitors, however, could be up to the job -- if they contained the right materials.

NC State physicist Dr. Vivek Ranjan had previously found that capacitors which contained the polymer polyvinylidene fluoride, or PVDF, in combination with another polymer called CTFE, were able to store up to seven times more energy than those currently in use.

"We knew that this material makes an efficient capacitor, but wanted to understand the mechanism behind its storage capabilities," Ranjan says.

In research published in Physical Review Letters, Ranjan, fellow NC State physicist Dr. Jerzy Bernholc and Dr. Marco Buongiorno-Nardelli from the University of North Texas, did computer simulations to see how the atomic structure within the polymer changed when an electric field was applied. Applying an electric field to the polymer causes atoms within it to polarize, which enables the capacitor to store and release energy quickly. They found that when an electrical field was applied to the PVDF mixture, the atoms performed a synchronized dance, flipping from a non-polar to a polar state simultaneously, and requiring a very small electrical charge to do so.

"Usually when materials change from a polar to non-polar state it's a chain reaction -- starting in one place and then moving outward," Ranjan explains. "In terms of creating an efficient capacitor, this type of movement doesn't work well -- it requires a large amount of energy to get the atoms to switch phases, and you don't get out much more energy than you put into the system.

"In the case of the PVDF mixture, the atoms change their state all at once, which means that you get a large amount of energy out of the system at very little cost in terms of what you need to put into it. Hopefully these findings will bring us even closer to developing capacitors that will give electric vehicles the same acceleration capabilities as gasoline engines."

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New Super-Earth Detected Within the Habitable Zone of a Nearby Cool Star

New Super-Earth Detected Within the Habitable Zone of a Nearby Cool Star

  10:12:00 AM    Science Lover

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An international team of scientists led by Carnegie's Guillem Anglada-Escudé and Paul Butler has discovered a potentially habitable super-Earth orbiting a nearby star. The star is a member of a triple star system and has a different makeup than our Sun, being relatively lacking in metallic elements. This discovery demonstrates that habitable planets could form in a greater variety of environments than previously believed.

An artistic conception of the two planets reported on in this
paper: b and c. Planet c is the one that lies in the habitable
zone of the star. Planet b is too hot to be habitable. (Credit:
Images courtesy of Guillem Anglada-Escud)

Their work will be published in The Astrophysical Journal Letters.

The team used public data from the European Southern Observatory and analyzed it with a novel data analysis method. They also incorporated new measurements from the Keck Observatory's High Resolution Echelle Spectrograph and the new Carnegie Planet Finder Spectrograph at the Magellan II Telescope.

Their planet-finding technique involved measuring the small wobbles in a star's orbit in response to a planet's gravity. Anglada-Escudé and his team focused on an M-class dwarf star called GJ 667C, which is 22 light years away. It is a member of a triple-star system. The other two stars (GJ 667AB) are a pair of orange K dwarfs, with a concentration of heavy elements only 25% that of our Sun's. Such elements are the building blocks of terrestrial planets so it was thought to be unusual for metal-depleted star systems to have an abundance of low mass planets.

GJ 667C had previously been observed to have a super-Earth (GJ 667Cb) with a period of 7.2 days, although this finding was never published. This orbit is too tight, and thus hot, to support life. The new study started with the aim of obtaining the orbital parameters of this super-Earth.

But in addition to this first candidate, the research team found the clear signal of a new planet (GJ 667Cc) with an orbital period of 28.15 days and a minimum mass of 4.5 times that of Earth. The new planet receives 90% of the light that Earth receives. However, because most of its incoming light is in the infrared, a higher percentage of this incoming energy should be absorbed by the planet. When both these effects are taken into account, the planet is expected to absorb about the same amount of energy from its star that Earth absorbs from the Sun. This would allow surface temperatures similar to Earth and perhaps liquid water, but this extreme cannot be confirmed without further information on the planet's atmosphere.

"This planet is the new best candidate to support liquid water and, perhaps, life as we know it," Anglada-Escudé said.

The team notes that the system might also contain a gas-giant planet and an additional super-Earth with an orbital period of 75 days. However, further observations are needed to confirm these two possibilities. "With the advent of a new generation of instruments, researchers will be able to survey many M dwarf stars for similar planets and eventually look for spectroscopic signatures of life in one of these worlds."

Anglada-Escudé was with Carnegie when he conducted the research, but has since moved on to University of Gottingen. His co-authors are Carnegie's Butler, Jeffrey D. Crane, Stephen A. Shectman, and Ian B. Thompson; Pamela Arriagada and Dante Minniti of Pontificia Universidad Catolica de Chile; Steve Vogt and Eugenio J. Rivera of University of California's Lick Observatory; Nader Haghighipour of the Institute for Astronomy & NASA Astrobiology Institute at University of Hawaii-Monoa; Brad D. Carter of University of Southern Queensland; C. G. Tinney, Robert A. Wittenmyer, and Jeremy A. Bailey of the University of New South Wales; Simon J. O'Toole of the Australian Astronomical Observatory; Hugh R.A. Jones of the University of Hertfordshire; and James S. Jenkins of the Universidad de Chile, Camino El Observatorio.

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